CED Spike2 Software v9 for Windows

Life Sciences Data Acquisition and Analysis System

Spike2 is a multi-channel continuous data acquisition and analysis package. Uses range from a simple chart recorder to complex applications requiring stimulus generation, data capture, scrolling or triggered displays, control of external equipment and custom analysis. This flexibility makes it ideal for applications in many fields including electrophysiology, neurophysiology, cardiovascular and respiratory studies.

Spike2 contains recording and analysis features suitable for a wide range of applications. Among the most popular features is it’s built in script language that can automate repetitive tasks and be used for customized protocols; and that it can import and export data to and from many common file types, including MatLab. These powerful data capture and analysis functions, together with one of the CED1401 family of laboratory interfaces, make Spike2 an extremely flexible, cost-effective and time-saving addition to any laboratory.

Features

• Navigate through your data quickly and simply using the intuitive user interface
• Record multiple data types, including waveforms, event times, coded markers, and discriminated single or multi-unit spike data
• Generate simple and complex protocols of waveform and pulse outputs
• Detect features automatically or manually and take measurements based on triggered and cyclical data
• Analyze multiple channels of waveform and event data on-line and off-line
• Display and export images as you want them
• Expandable by synchronizing multiple CED 1401s
• Replay data files, with simultaneous output of waveform data

Data Acquisition Using a CED 1401

• Set different waveform sample rates per channel
• Capture and classify single and multi-unit spike activity in real-time
• Continuous, internally timed and triggered recording modes
• Record multimedia video and sound, time-locked to Spike2 data
• Store up to 8 channels of event data with microsecond timing resolution
• Log accurately-timed coded 8-bit digital inputs
• Annotate data records with text notes and keyboard markers
• Calibrate waveforms with multiple methods including values, areas, slopes
• Automatically save and sequence multiple files with optional trigger start
• Recover data files in the event of an un-commanded system shutdown

Processing and Analysis

• Waveform analyses including averaging, power spectra and waveform correlations
• Event analyses including INTH, PSTH, auto and cross correlations and phase histograms
• Automatically find data features in evoked, spontaneous activity and cyclical data
• Generate XY plots and data measurements based on cursor feature detection measurement values
• Process waveforms dynamically on- and off-line.
• Digitally filter waveforms (FIR and IIR) with interactive filter design -
• Interactively fit data with functions including exponential, Gaussian, polynomial, sinusoid and sigmoid

Examples of Spike2

Multi-channel on-line spike discrimination with 'spike monitor' display>

Script driven detection and classification of seizure activity in EEG

Response of insect auditory receptor to pure tone stimuli and calling song

Scripted sleep stage scoring and analysis